Vol. 12, Issue 4 (2023)

Author(s):
Esha Sinha, Salauddin Qureshi, Richa Sarkar, Ambika Arun, Krishna J Patel, Venu Gorre, Khushboo Panwar, Sudhir Kumar Prajapati, Satyabrat Dutta, Bhavani Puvvala, Sabita Behera, Manimaran N, Y Sriharsha and Subuhi Khan

Abstract:
Opportunistic mycoses cause a wide range of diseases in animals and birds, from localized infections to fatal disseminated diseases, such as aspergillosis, candidiasis, cryptococcosis, and infections especially caused by mycotoxins. The manipulation of fungal genomes is critical for understanding the function of target genes, pathobiology of fungal infections, virulence potential, and pathogenicity of clinically important fungi, as well as the development of new diagnostics and therapeutic targets. In this review, we provide a thorough introduction to the CRISPR/Cas9 system and a summary of the most recent fungal genome editing applications using CRISPR/Cas9. This technology has been successful in elucidating the pathogenesis of fungal species and the interactions between hosts and pathogens. It has also produced novel insights into the susceptibility to and resistance to antifungal drugs, as well as creative treatment plans and an increase in the secondary metabolite content of fungi.