Vol. 12, Issue 3 (2023)

Author(s):
Madhavi Khilari, Heera Lal, Megha Kashyap, Shashi Kiran Minj and Lilagar Singh Verma

Abstract:
The present investigation was done on impact of different hardening substrates on growth of in vitro raised plantlets with objective to standardize hardening media for acclimatization of orchid plantlets. The research was laid out in Completely Randomized Design (CRD) in combination of 16 treatments containing charcoal, brick, cocopeat, bark, rice husk, groundnut shell, chopped coconut husk and chopped paddy straw in different combination all 16 treatments replicated thrice for analyzing the effect of media under poly house condition. Among the treatments, T₈ (chopped coconut husk + brick + charcoal + cocopeat) had superior result in almost all considerable aspects of study than all other treatments including control. The results revealed that combination of chopped coconut husk, brick, charcoal and cocopeat had been beneficial to significantly increase the vegetative growth and can be recommended for hardening media of orchids.