Vol. 12, Issue 2 (2023)

Aim: To determine the phytochemical composition and antimicrobial properties of tamarind extracts on Fusarium oxysporum pathogen of Azadirachta indica diseased leaves.

Study Design: Completely Randomized Design (CRD).

Methodology: The phytochemical constituents in ordinary, warm and hot water as well as Methanolic extracts of tamarind pulp were screened. The Zone of Inhibition (ZOI) diameter (mm), Minimum Inhibitory Concentration (MIC) against Fusarium oxysporum pathogen were determined. Data were analyzed using ANOVA at P = 0.05.

Results: The Methanolic tamarind fruit pulp extract was obtained using the Soxhlet extraction method. The total number of compounds identified in methanol extract tamarind pulp was 10. The major phytoconstituents present in the methanol extract of Tamarindus indica L were Tannins, saponins, flavonoids, carbohydrates, reducing sugar, Glycosides, Alkaloides and proteins. Test for Anthraquinones and phytosterols was negative which indicates that they are absent in the methanolic extract of Tamarind pulp. Antimicrobial activity was carried out against the pathogen Fusarium oxysporum which Identified from Azadirachta indica, infected leaves through morphological evidence. The results represented as the antimicrobial activity of the tamarind extracts against Fusarium oxysporum at different concentrations at 25%, 50%, 75%, 100% and positive control referred to as treatments T₁, T₂, T₃, T₄, and T₅, the maximum zone of inhibition was observed at Positive Control i.e., T₅ (15.00 mm) which is more than the extracts which obtained from Tamarindus indica and T₄ is 100% concentration of crude is (13.66 mm) followed by (12.00 mm) at 75% of T₃, (8.00 mm) at 50% of T₂, and minimum inhibition was at 25% of T₁ (5.6 mm). Due to the presence of phytochemicals this zone of inhibition may be resulted. Minimum zone of 5.00 mm was observed in Fusarium oxysporum at 25% dose and a maximum of 15.00 mm zone at positive control T5 dose.