Pharmacokinetics of levofloxacin through oral route of administration in dual purpose chicken by the LCMS/MS analytical technique
Ravikumar C, Jagadeesh S Sanganal, Shridhar NB, Sunilchandra U, Narayanaswamy HD, Ramachandra SG and Shivashankar BP
Levofloxacin, a third-generation fluoroquinolone, is the S-isomer of ofloxacin and possesses excellent activity against gram-positive, gram-negative and anaerobic bacteria (North et al., 1998). The study was carried out to evaluate the pharmacokinetic parameters following single oral dose administration of levofloxacin in dual purpose chicken. The study was conducted in 30 to 40 day old healthy dual purpose chicken Indian Rock -3 (IR-3), a strain of White Plymouth Rock developed by Karnataka Veterinary Animal Fisheries Sciences University, Bidar. The study was performed at the department of Poultry Science, Veterinary College, Hebbal, Bengaluru. In the pharmacokinetic study, experimental birds were (n= 10) were administered with levofloxacin at 10 mg/ kg bw single dose through oral route directly in to crop using a thin plastic tube attached to a syringe. Following oral administration, blood samples were collected at time 0 min, 5 min, 10 min, 15 min, 30 min, 45 min, 1 h, 2 h, 4 h, 6 h, 8 h, 12 h, 16 h and 24 h. Plasma was separated soon after blood collection by centrifugation (8000 rpm at 4 °C for 10 min), stored at -20 °C until the analysis of the pharmacokinetic parameters. The determination of the pharmacokinetic parameters like AUC0-24 (μg/ml/h), AUC0-∞ (μg/ml/h) AUMC0-24 (μg/ml/h), Cmax (μg/ml), C0p (μg/ml), Tmax (h), MRT (h) Oral bioavailability (F)%, Vd (L/kg), Biological t1/2(h), and Total body clearance (L/kg/h) by the LCMS/MS analytical technique through the non compartmental analysis PK solver soft ware system. In the present study, there was an increase in the values of AUC, AUMC, Cmax, C0p, t1/2 and MRT pharmacokinetic parameters after oral administration compared to the previous studies in the dual purpose chicken.
How to cite this article:
Ravikumar C, Jagadeesh S Sanganal, Shridhar NB, Sunilchandra U, Narayanaswamy HD, Ramachandra SG, Shivashankar BP. Pharmacokinetics of levofloxacin through oral route of administration in dual purpose chicken by the LCMS/MS analytical technique. Pharma Innovation 2023;12(2):30-39.