Vol. 12, Issue 2 (2023)

Author(s):
MA Bhosale, SV Sawardekar, UB Pethe, SG Mahadik, MG Palshetkar, SD Patil and SB Rupannavar

Abstract:
Farmers are hesitant to plant pigeon pea crop due to a lack of irrigation infrastructure during the rabi season because the crop lasts more than 180 days. As a result, there is a need for pigeon pea genotypes with early maturity (150 days). Present investigation meant to evaluate genetic diversity and variability of M3 generation of pigeon pea through ISSR markers where laboratory work performed at Plant biotechnology Centre, College of Agriculture, Dapoli. Phenotypically 50 mutants were selected for growing further generation on the basis of yield and yield contributing characters. Variability of 50 selected mutants was confirmed through molecular markers which are not affected by environment. DNA isolation was done by using CTAB method. 20 ISSR primers were showed polymorphism. UBC 885 recorded maximum polymorphism percent i.e., it shows more variation. Primer UBC 886 exhibited minimum polymorphism percentage. The dendrogram demonstrated that mutants. T1(21)7-8 and T1(67)11-5 had relatively more genetic distances over the control (Konkan Tur-1), i.e., 0.505 bp and 0.470 bp, respectively, while T1(77)9-1(0.095 bp) and T2(36)15-2(0.058 bp) had a few genotypic distances with respect to control.