Vol. 12, Issue 2 (2023)

Author(s):
Garima Kansal, Hari Om Pandey, Sadhan Bag, Kuldeep Kumar and Triveni Dutt

Abstract:
The bone marrow contains a rare population of cells termed mesenchymal stem cells having the ability to differentiate into cells of the osteogenic, chondrogenic and adipogenic lineages. There is considerable interest in utilizing MSCs in a broad repertoire of cell-based therapies for the treatment of various diseases. Therefore, rapid and simple method for culture of MSCs is required. Here we describe method for the isolation and in vitro expansion of a highly enriched population of MSC from mouse cortical/compact bone. The procedure includes flushing bone marrow out of the long bones in complete α-MEM medium. This was followed by two times washing in complete α-MEM medium and finally plating in tissue culture flasks. After 2 days of primary culture, non adherent cells were removed by changing the medium at every third day. Once the cells reach 70-90% confluence, the cells were passaged further to augment cell population. Identification assays confirmed that these cells were MSCs, which may be used for therapeutic purpose.