Vol. 12, Issue 2 (2023)

Present investigation aimed to banana (CV. Grand Naine) optimize medium combination for callus induction and in vitro regeneration from male floral organ of banana (CV. Grand Naine). The study was conducted in Plant Biotechnology Centre, Dr. Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, Dist. Ratnagiri (M.S) during the academic year 2020-2022. The various sized explants from male flower bud were transferred to MS medium containing different combination of PGRs. Among the various combination highest frequency of callus induction (86.00%) was observed in MS medium supplemental with MS + 2.5 mg/l 2,4-D+0.5mg/l-1+BAP+NAA. During development of callus different types of calli with variation in colour and texture were noted. Highest frequency of shoot regeneration from callus was reported in MS medium supplemented with 4.5 mg/l BAP. In vitro regenerated shoots successfully rooted (80%) in full strength MS medium fortified with 0.5 mg/l NAA + 1.5 mg/l IBA. In vitro regenerated plantlets were transferred to the pots with 80% success rate.