Vol. 12, Issue 10 (2023)

Author(s):
Raut Pooja and Ingle RW

Abstract:
Ralstonia solanacearum, a devastating phytopathogenic bacterium, poses a significant threat to global agriculture, particularly in crops like chilli (Capsicum annum). This study aimed to isolate and biochemically characterize Ralstonia solanacearum strains associated with chilli plants. Chilli plants exhibiting typical symptoms of bacterial wilt, including wilting, leaf yellowing, and vascular discolouration, were sampled from multiple regions. Isolation of the bacterium was achieved through culture-dependent techniques on selective media. Subsequently, the isolated strains were subjected to a comprehensive biochemical characterization, including Gram staining, KOH test, Catalase test, Starch hydrolysis test, Lipase activity on Tween 80 Agar, Hugh-Leifson test, Gelatin liquefaction test, Trypton Glucose Extract Agar test, Triphenyl Tetrazolium Extract Agar test. The result revealed the successful isolation of Ralstonia solanacearum strains from affected chilli plants.