Vol. 12, Issue 1 (2023)

Author(s):
Pasupuleti Pranuthi, Dr. T Suseela, Dr. K Ravindra Kumar, Dr. AVD Dorajee Rao, Dr. DR Salomi Suneetha, Dr. K Umakrishna and Dr. M Paratpara Rao

Abstract:
Success of a commercially viable protocol for mass multiplication of plants using tissue culture begins with effective elimination of microbial contamination by pre-treatment and surface sterilization methods. In the present study, for development of axenic culture from axillary buds of tuberose. Among the various pre-treatment combinations tested, minimal microbial contamination and maximum survival percentage was found when the explants were treated with a combination of Carbendazim 0.1% + Mancozeb 0.1% + Streptomycin 100 ppm + Teepol 1% for overnight. Surface sterilization was achieved at the best by treating the pre-treated explants with NaOCl 4.0% (15 min.) + 75% ethanol (1min.) + HgCl₂ 0.1% (10 min.). Among the various treatments tested for culture establishment, the results were recorded when the explants were placed in MS + 2.0 mg L^-1 BAP + 0.1 mg L^-1 NAA.