Vol. 12, Issue 1 (2023)

Author(s):
Shubhnish, VS Panwar, BS Tewatia and Jyotsana

Abstract:
The present study was planned to investigate Nutrigenomic expression level of TLRs (Toll-like Receptors), viz. TLR 2, TLR 4 and TLR 7 gene transcripts of broiler chickens when supplemented with different sources of zinc. One hundred and sixty day-old broiler birds were equally and randomly distributed into four treatment (T₁, T₂, T₃ and T₄) groups which were subdivided into four replicates containing ten birds in each, reared on deep litter system up to 6 weeks of age. T₁ group was kept as control and offered maize-soybean meal based diet without any source of zinc in mineral mixture. Treatment groups T₂, T₃ and T₄ were supplemented with inorganic, organic and Nano zinc, respectively. The Nutrigenomic expression data revealed that mRNA expression of TLR 2 and TLR 7 of broilers was enhanced in all the groups supplemented with different forms of zinc, either inorganic, organic or Nano, with a highest up regulation in the Nano zinc supplemented group. A down regulation pattern was observed in the RQ (Relative Quantification) values of TLR 4 with a minimum RQ value in Nano zinc supplemented group when compared with other treatments. The present study revealed that Nano zinc supplementation had a stimulatory effect on the up regulation of TLR 2 and TLR 7 and down regulation of TLR 4 indicating good immunity and healthy gut microflora.