Vol. 11, Issue 8 (2022)

Author(s):
Chandana J, TSKK Kiran Patro, Dr. Salomi Suneetha, K Usha Kumari, M Paratpara Rao and K Aruna Kumari

Abstract:
The present investigation entitled “Standardization of surface sterilization for in vitro propagation of potato (Solanum tuberosum L.) Variety Kufri Surya” was conducted at Plant Tissue Culture Laboratory, College of Horticulture, Dr. Y.S.R Horticultural University, Andhra Pradesh in a Completely Randomized Design. Sprouts were pretreated and surface sterilized with 0.1% mercuric chloride at 4, 6 and 8 minutes and sodium hypochlorite at 5,10 and 15 minutes. Comparison was done between two important sterilants mercuric chloride and Sodium hypochlorite. The observations recorded regularly at 7 days for the fungal, bacterial, total contamination, percent mortality other than microbes and explant survival. Results revealed that between two sterilants, HgCl2 was found better for controlling the contamination though prolonged treatment showed adverse effect on explants due to chemical toxicity causing browning. Mercuric chloride (HgCl2) at 8 min was selected was found as best suitable sterilization chemical as it reduces contamination and increases explant survival.