Vol. 11, Issue 7 (2022)

Author(s):
Sujata N Dandale, SS Mane, ST Ingle, AN Patil, RS Nandanwal and PV Jadhav

Abstract:
The Chickpea wilt, one of the most important fungal pathogen of chickpea, is a constant threat to this crop plant. In the present study gene expression analysis of chickpea roots during Foc infection was performed using various approaches viz. cDNAs derived from total mRNA during infection process of susceptible (JG-62) and moderately resistant (Jakki-9218) and resistant (WR-315) cultivars, were amplified using random oligonucleotides. The sequence characterization of differentially expressed transcripts revealed their homology with many plant genes essential for various metabolic functions including defense. Further, expression patterns of specific candidate gene transcripts were analyzed in the Foc inoculated and uninoculated resistant moderately resistant and susceptible chickpea cultivars, on day 7^th and 14^th days of infection. The semiquantitative RT-PCR analysis of defense related genes was performed using gene specific oligonucleotides in resistant moderately resistant and susceptible chickpea cultivars. The expression of fungal pathogenesis related genes and their race specific response was determined throughout the course of chickpea- FOC interaction. Temporal expression and race specific response of plant defense related and fungal virulence genes were studied in the resistant and susceptible cultivars of chickpea inoculated with three races of Foc highlighting the host-pathogen interactions. Few genes, involved in chickpea defense against Fusarium wilt which was not reported previously were unveiled in this study.