Vol. 11, Issue 6 (2022)

Author(s):
Arti Wahurwagh, AG Deshmukh, Dipika Padole, PV Jadhav and MP Moharil

Abstract:
Sarpagandha is a medicinal shrub. It has been used in Ayurveda from ancient times. Nodal segment used on Murashige and Skoog medium. Sterilization of explants done with bavistin, streptomycin, NaOCl and HgCl2. MS+ BAP and IBA proved more responsive and effective for proliferation than BAP alone. MS+ 5 mg/l BAP+ 0.5 mg/l IBA found significantly better with 5.52 shoots and 81.30% shoot proliferation. Explants were slightly more responsive in the media combination MS+ 5 mg/l BAP+ 1.0 mg/l IBA with ±6.53 days and maximum length of shoots (6.93 cm). Root induction found best in ½ MS media+ NAA+ IAA, responded in ±29.3 days showing maximum root length. 1:3 soilrite and cocopeat hardening media with watering after 1 day of planting gave maximum survival in primary hardening. Present investigation indicates that, in-vitro regeneration protocol will provide techniques that can be successfully used for mass in-vitro propagation of species, Rauwolfia serpentina L.