Vol. 11, Issue 6 (2022)

Author(s):
V Venkanna, Y Hari, K Rukmini Devi, G Shravan and T Shasthree

Abstract:
Pollen fertility is restored by nuclear-encoded genes called fertility restorer (Rf) gene. For developing high yielding heterotic hybrids, the first step is to identify restorers that can efficiently restore the fertility of CMS lines. As the fertility restoration of CMS-WA lines is principally controlled by two independent and dominant nuclear fertility restoring genes, Rf3 and Rf4. In the present study, we made an attempt for genotyping of 25 rice genotypes for the presence of Rf3 and Rf4genes by using functional markers. Out of 25 rice genotypes, we observed that six genotypes namely RR 32, RR 49, WGL 616, WGL 639, WGL-674& WGL 1068 were observed to be double positives for Rf3 and Rf4genes and hence these five genotypes may be used as male parents or restorers in CMS based hybrid rice breeding programs.