Vol. 11, Issue 5 (2022)

Author(s):
V Deepashree, M Ganga, M Jawaharlal, S Manonmani and M Suganthy

Abstract:
Jasminum auriculatum is flower crop widely cultivated as loose flower. The crop has slight difficulty improvement as it has narrow genetic variability hence induction of mutagenesis through gamma rays and EMS application has been adopted to create variability in jasmine. Therefore, the objective to evaluate the putative mutants of J. auriculatum generated through physical and chemical mutation with various dosages of gamma radiation and EMS at M₁V₁ and M₁V₂ generation. The cuttings were irradiated with 10, 15, 20 and 25 Gy of gamma rays and with EMS (25, 30, 35 and 40 mM) for M₁V₁ generation and individual plant parameter were observed in both M₁V₁ and M₁V₂ generation. Data were collected on plant height (cm), number of primary branches (cm), internodal length (cm), number of leaves, leaf area (cm²), days of flowering, flower bud length (cm), corolla tube length (cm). High doses of gamma radiation lower germination and survival percentages, according to field observations of M₁V₁ generation. The LD₅₀ (50 percent lethality) was attained at 20 Gy and 40 mM. The morphological parameters were all affected with increase in dosage with gamma rays and EMS. The plant height and internode length seemingly decrease with increase in the dosage. Whereas M₁V₂ generation the survival rate differed depending on the x-ray irradiation dosage, as well as the uneven establishment count after planting were observed. Persistent physiological damage transferred from M₁V₁ and M₁V₂ generation caused the survival rate to drop. Observation on growth and flowering character showed positive effect on the flower bud length (3.10 cm), corolla tube length (2.42 cm) and the number days to flowering was higher M₁V₂ generation. The mutant’s morphological differences from the parental plant (control) indicated that new genetic diversity had emerged in the irradiated J. auriculatum.