Molecular and field level screening for blast resistance among various rice varieties in two blast hotspots of Chhattisgarh
Anushree Pramanik, AK Sarawgi, Sonali Kar and Rakhi Dubey
Rice blast, caused by Magnaporthe oryzae, is becoming one of the most ruinous ailments of rice (Oryza sativa L.) in Chhattisgarh. Utilization of resistant rice varieties had been demonstrated to be the most effective and sustainable way to command this disease. In the present study, the existences of blast resistance genes were identified in some of the rice genotypes in field level screening. The selected genotypes for screening were confirmed for blast resistance genes in some of the lines. The material was consisted of rice lines containing germplasm lines, advanced breeding lines, land races and blast resistant varieties. These lines were identified using the field level screening respectively and further confirmed through SSR markers RM224, RM 72 and RM206. Results showed the presence of gene Pi 1 in nine varieties, Pi33 in three varieties and Pi kh in five varieties, some varities were with combination of genes against blast resistance. Infection index analysis after field level screening also showed that ten varieties had low infection index. Landraces carrying the genes Pi 1 and Pi kh imparted moderate resistance under Chhattisgarh conditions which implied that pyramiding of these genes could improve the disease resistance in rice varieties of Chhattisgarh. Among the selected varieties IR64 and Tetep having Pi33, Pi 1 and Pi kh genes could be used as gene donors in further breeding programmes.
How to cite this article:
Anushree Pramanik, AK Sarawgi, Sonali Kar, Rakhi Dubey. Molecular and field level screening for blast resistance among various rice varieties in two blast hotspots of Chhattisgarh. Pharma Innovation 2022;11(4):2110-2112.