Abstract: The present investigation was meted out on two promising sugarcane genotypes CoN-13072 and CoN-13073, were exposed to different NaCl levels to assess rhizobacterial interaction for salinity tolerance. From the shoot apex portion of two sugarcane genotypes tissue culture plantlets were developed. From that regenerated plantlets shifted to polyhouse and exposed to different NaCl levels to assess rhizobacterial interaction for salinity tolerance. Maximum shoot length (25.40 cm) was observed in genotype CoN-13073 under rhizobacterial inoculum at 2.0% NaCl concentration and maximum root length (12.40 cm) was observed at 0.0% and 1.5% NaCl concentration under rhizobacterial inoculum in genotype CoN-13072 and CoN-13073 respectively. Maximum shoot root ratio (2.60) and (2.40) was observed at 0.0% NaCl concentration with rhizobacterial inoculum within the rooting mixture of both the genotypes CoN-13073 and CoN-13072 respectively. High chlorophyll content index (5.48) and leaf area (41.20 cm2/plant) was observed in genotype CoN-13073 at 0.0% NaCl concentration under rhizobacterial inoculum. In genotypes CoN-13073 and CoN-13072, with a rise in NaCl concentration upto 2.0% and 1.5% respectively, increase in shoot length, leaf area and chlorophyll content index was observed as a result of increase in ACC deaminase activity. ACC deaminase activity and ethylene content within the plant body are negatively correlated. Increase in ACC deaminase activity results decrease in ethylene synthesis in plant body to avoid stress. Among the 2 genotypes, CoN-13073 found to be more salt tolerant at higher NaCl levels with the exhibition of low osmotic conductance, maximum leaf area with high chlorophyll content index.