Vol. 11, Issue 3 (2022)

Author(s):
Deepak Kumar, Vikram Singh, Shikha Yashveer, Samita, Kiran, Ganesh Kumar Koli and Om Prakash Yadav

Abstract:
Genetic diversity among nine wheat cultivars was evaluated using 23 polymorphic microsatellite (SSR) markers. Analysis of molecular diversity revealed moderate levels of polymorphism in the set of genotypes studied. Fifty six alleles with an average of two alleles per locus were detected. The allelic polymorphism information content (PIC) value ranged from 0.16 to 0.67 with an average of 0.33. The primers like gwm192, gwm111, gwm374, STSS5765, XPSP3000, wmc104 and gwm192 could be considered particularly informative, as they revealed three or more alleles per locus and displayed high PIC values. The most closely related cultivars were ‘WH1184’ and ‘WH1124’ displaying the lowest dissimilarity index, whereas highest genetic distance was observed between ‘WH711’ and ‘HD3059’. The dissimilarity indices ranged from 0.62 to 0.85. Three major clusters comprising one, one and seven genotypes were formed. The three clusters and their similarities might assist breeders to set up the appropriate guidelines for successful breeding of wheat cultivars based on the established relationships.