Vol. 11, Issue 2 (2022)

Author(s):
Hema, Anjana Nautiyal, Prerna Negi, Anita Trivedi and Himani Guleria

Abstract:
The Experiment work was conducted at High Altitude Plant Physiology Research Centre (HAPPRC), HNBGU, Srinagar Garhwal, and Uttarakhand, India. Ten months old plant parts were used as explant. The tissue culture work was done with the plant of Ashwagandha (Withnia sominfera) which is one of the major herbal components of geriatric tonics mentioned in Indian systems of medicine. It is greatly use in Ayurveda. The work was started with the media preparation, domestic sugar and sucrose were used with the different concentrations of PGR. Inoculated all the explants in the prepared media with different hormonal concentrations under the aseptic condition. Swelling of the explants was observed one week after inoculation. However, callus initiation was observed in the second week after inoculation. Four weeks later, callus initiated at the cut edge of the explants and developed into a full grown callus. The callus was morphologically found to be yellowish-white, compact, dry and nodular. The result was observed that the domestic sugar and sucrose with the concentration of 3µM BAP gave better response respectively. The callus (3µM BAP) was sub cultured again with the different concentrations of BAP and observed high rate of callus mass increased in 3µM and 5µM of BAP.