Vol. 11, Issue 2 (2022)

Author(s):
Sunaina Varma, Data Ram Kumhar, Priyanka and Mukesh Kumar Sheshma

Abstract:
Efficacy of antagonists and fungicide were evaluated under in vitro conditions for the mycelial growth inhibition. The antagonistic actions of three species of Trichoderma and two spp. of bacteria were evaluated against the test fungus by dual culture and paper disc technique, respectively. It was observed that all the biocontrol agents were significantly superior in inhibiting the mycelial growth of test fungus over control. Maximum growth inhibition was recorded in T. harzianum (64.81%) followed by T. viride and T. atroviride. Among the bacterial bioagent Pseudomonas fluorescens inhibit (41.48%), this was statistically at par with Bacillus subtilis (34.81%). The efficacy of Tebuconazole 2DS on mycelial growth of R. solani was recorded after 3, 5 and 7 days with the concentrations of 1.0, 10, 25, 50 and 100 ppm. The maximum inhibition of mycelial growth was observed in100 ppm where it was 16.66 mm, 17.33 mm after 3, 5 and 7 days respectively followed by 50 ppm where it was 34 mm, 35.70 mm and 36.33 mm after 3, 5 and 7 days, respectively. While 10 ppm and 1 ppm concentration was not much effective and it was descending order of merit. Thus 100 ppm and 50 ppm concentration effectively check the mycelial growth of R. solani in all the growth stage period i.e. 3, 5 and 7 days.