Vol. 11, Issue 2 (2022)

Author(s):
Sunaina Varma, Data Ram Kumhar, Priyanka and Mukesh Kumar Sheshma

Abstract:
In the present investigation, in vitro effect of different temperature, different carbon sources, nitrogen sources and effect of different hydrogen ion concentration were evaluated against Rizoctonia solani Kuhn in which mycelial growth was observed and numbers of sclerotia were counted. Among the different temperature i.e. 20 ^oC, 25 ^oC, 30 ^oC, 35 ^oC and 40 ^oC, maximum mycelial growth and sclerotia was observed at 30 ^oC. Among the different carbon sources i.e. glucose, sucrose, maltose, fructose and lactose maximum mycelial growth and sclerotia were recorded in sucrose source. Similarly different nitrogen sources i.e. ammonium chloride, L-alanine, L-agrinine, glutamic acid and ammonium nitrate, most suitable growth media was ammonium nitrate in which maximum mycelial growth and maximum number of sclerotia were recorded and among the different hydrogen ion concentration (pH) levels i.e. 6.0, 6.5, 7.0, 7.5 and 8.0 most suitable pH was 7.0 in which maximum mycelial growth and sclerotia were observed.