Vol. 11, Issue 2 (2022)

Author(s):
Dinesh Singh Dhurwey and Jayant Bhatt

Abstract:
A random survey of tribal areas of Madhya Pradesh viz., Anuppur Dindori, Mandla, shahdol and Umaria districts was conducted during 2016-17 to find out the infestation of root knot nematode in wheat and paddy fields. The soil samples were collected from the infested fields and the samples were analysed using Baermann’s funnel technique followed by Cobb sieving and decanting methods. The populations of Meloidogyne graminicola were morphometric and morphological identified using preparation of perineal pattern and comparisons were made for various nematode life stages of nematode populations were similar to M. graminicola on the basis of length and width of egg, juvenile, male and female high line tail portion, distance up to excretory pore EPST (distance of excretory pore from anterior end / stylet length female) ratio and vulval length. In the present investigation, molecular identification was conducted using ITS based techniques applying for four samples collected from viz., Anuppur, Dindori, Shahdol and Umaria. All the four samples produced around 450bp intact band after PCR with ITS1 and ITS4 forward and reverse primer pairs. After sequencing of PCR product, the blast X analysis of sequences depicted 100 percent homology with M. graminicola in all four samples.