Vol. 11, Issue 2 (2022)

Author(s):
Gawande SM, Ghuge SB, Kalpande HV and Wankhade MP

Abstract:
The present investigation was undertaken to study the effect of mutagens on emergence, plant survival and pollen sterility in PBNS-12 variety of safflower (Carthamus tinctorius L.) in M₁ generation. The seeds of safflower variety PBNS-12 were treated with different doses of gamma rays viz., 100 Gy, 200 Gy, 300 Gy, 400 Gy, 500 Gy and different concentration of EMS viz., 0.1%, 0.2%, 0.3%, 0.4% and their combination treatments. The observations were recorded on emergence percentage, plant survival percentage and pollen sterility percentage in M₁ generation. Results revealed the significant effects of mutagen dosages and treatments periods on emergence, plant survival and pollen sterility for all the mutagens tested. In the present study, the seed emergence and plant survival rate decreased with increased in doses/ concentrations both in gamma rays and EMS and their combination treatments. The pollen sterility rate increased with an increase in dose /concentrations of both gamma rays and EMS and their combinations treatments in M₁ generation.