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Vol. 11, Issue 2 (2022)

Optimization of doses for Ethyl Methane Sulphonate (EMS) and analysis of M1 generation of fodder cowpea [Vigna unguiculata (L.) Walp]

Author(s):
Arya S Nair and Gayathri G
Abstract:
Mutation breeding is an important tool for creating variability and the source of new breeding materials. Ethyl Methane Sulphonate (EMS) is a very potent chemical mutagen. The present study envisages the optimization of doses of mutagen for fodder cowpea and analysis of M1 generation. Fodder cowpea seeds were treated with different concentrations of EMS (0.1%, 0.2%, 0.3%, 0.4% and 0.5%) and lethal dose 50 (LD50) value was determined using probit analysis. Effective doses were fixed according to LD50 value. Analysis of M1 generation was carried out on cowpea, treated with effective doses of mutagen. Germination parameters like seedling shoot length, root length, vigour index and the quantitative characters like number of pods/plant, pod length, number of seeds/plant and seed yield/plant and presence of chlorophyll mutants were recorded from M1 generation. All the biometric characters showed wide variation among the mutant population. Chlorophyll mutant plants were observed in all mutated populations. Presence of chlorophyll mutants were recorded higher in 0.51% of EMS treatment. Generally, a reduction in the quantitative parameters was noted with the increase of concentration of the mutagen.
Pages: 593-598  |  269 Views  85 Downloads


The Pharma Innovation Journal
How to cite this article:
Arya S Nair, Gayathri G. Optimization of doses for Ethyl Methane Sulphonate (EMS) and analysis of M1 generation of fodder cowpea [Vigna unguiculata (L.) Walp]. Pharma Innovation 2022;11(2):593-598.

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