Vol. 11, Issue 2 (2022)

Author(s):
Anju Nehra, Jagdish Singh and Rajesh C Jeeterwal

Abstract:
The present investigation was under taken to the study of Response of different culture medium on in vitro plant regeneration through different explants viz., Cotyledons, Stem segment and Leaf base in Ishwarmul (Aristolochia indica Linn). For In-Vitro regeneration of Ishwarmul, seven media were tested out of which five based on Murashige and Skoog’s medium (MS), one on Gamborg’s B₅ and one on White’s media for their response on induction of callus from Stem segment, Leaf base and Cotyledons. Media based on MS responded well irrespective of explants used. Leaf base proved to be the best for callusing percent and fresh weight of callus in MS based media. The highest callusing efficiency was observed in MS medium with full strength of MS salts, 5mg/l 2,4-D + 1.6mg/l BAP. Observation on shoot regeneration capacity suggested that MS medium contained MS salt +5mg/l 2,4-D+1.6 BAP has shown superior performance and was highly effective in inducing multiple shoots from callus. The MS medium with half strength of basic MS salts in combination with 1.2mg/l BAP/ 0.6 mg/l IBA was also found to be the best for root regeneration. The Darkness supports the fast root regeneration in Ishwarmul. Three explants viz. Cotyledons, stem segment and leaf base were evaluated for their effectiveness for induction of callus on different media. The leaf base was proved as the best explants on the basis of callusing percentage and averaged fresh weight of callus. Least effective explants were cotyledon. The evaluation of media for shoot regeneration capacity suggested that medium M₃ contained MS salt +5mg/l 2,4-D+1.6 BAP has shown superior performance of M₃ medium may be due to the presence of high concentration 2,4-D and BAP which are supported to be highly effective inducing multiple shoots from callus. The rooted plantlets were successfully transplanted in pots in poly house, after 25 days these plants with pots were transferred in to green house for further acclimatization under natural environments. The survival rate was 63%.