Vol. 11, Issue 2 (2022)

Author(s):
Uttara Sahu, D Dash, Gayatri and Karthika P

Abstract:
The experiment was being carried out in the summer season, 2020-2021 at College of Agriculture, Raipur, Chhattisgarh to study the dual inoculation of stress tolerant Rhizobium and Phosphorus solubilizing bacteria (PSB) on the growth and yield of mung bean crop. The experiment was laid out in RCBD with nine treatments replicated thrice. Rhizobium and PSB isolates collected from culture deposits of Department of Agricultural Microbiology, IGKV, Raipur were studied for screening of their stress tolerance ability.The effect of pH and temperature on Rhizobium and PSB isolates were tested by growing strains on YEMA and PVK medium respectively. Isolates were subjected to extreme acidic pH (pH: 4.5) and extreme temperature (55 ⁰C), Two isolates each from Rhizobium (Mung 8 and Mung 3512) and PSB (PSB 5-69 and PSB 5- 71) were screened as stress tolerant isolates which found favourable colony growth at low pH (4.5) and high temperature, hence used for inoculation as single and dual inoculation in experiment with mung crop. Results showed that dry biomass of shoot as well as shoot N uptake were observed for significant increase i.e., 0.93 to 2.68g/plant and 16.00 to 70.22 mg N/ plant respectively in co- inoculation of isolates, Mung8 and PSB-5-69 over single inoculation and control at 40 Days after sowing. Grain yield also increased significantly (7.28 to 12.78 qha^-1) by application of treatment T₆(Mung8+PSB-5-69) followed by T₈ (Mung8+PSB-5-71) over uninoculated. Combined application of Mung8 and PSB-5-69 was considered to be the suitable treatment and can be recommended as nutrient management strategy for yield enhancement under stressed condition.