Vol. 11, Issue 12 (2022)

Author(s):
Akhil Sugunan, Veena Vighneswaran and Deepa Thomas

Abstract:
The study was focused to obtain reliable estimate of genetic fidelity with respect to “number of subcultures” followed by comparing qualitative, quantitative and molecular level validation. Hardened “Robusta (Musa AAA)” plants derived from different multiplication stages namely; S6, S7, S8, S9, S10 and S11 were the experimental material used in the study. Stages of subculture were considered as different treatments as the study was mainly focused to limit the shoot multiplication stages to optimum subculture levels which ensures farmers demand of “clonal uniformity”. Field analysis was performed using RBD with six treatments (S6, S7, S8, S9, S10 and S11) and four replication at Rice Research Station, Vyttila, Kerala and qualitative and quantitative comparisons were done. Molecular analysis was performed using DNA isolated from leaves of experimental plants. DNA isolated from sucker of mother plant used for initial culture establishment, represented as “S0” was the reference to confirm genetic uniformity. In order to compare variation pattern in field with molecular level, ten newly developed SSR markers having high homology with predicted genes were used and possible molecular interpretations were suggested.