Vol. 11, Issue 12 (2022)

The present study was aimed towards the identification of single nucleotide polymorphism in Sahiwal cattle using the reduced representation sequencing method. Blood samples were collected from ten unrelated Sahiwal cattle, DNA isolated and sequenced using ddRAD approach. After processing of sequenced data using various bioinformatics tools, a total of 279383 SNPs were identified in Sahiwal cattle genome with reference to the Bos taurus genome, and upon annotation, nearly half of the total variants were found to be in the intronic region (49.25%), followed by the intergenic region (41.25%). The SNPs identified in this study may serve as molecular markers for economically important traits and may be used in future breed improvement and conservation programs.