Vol. 11, Issue 1 (2022)

Author(s):
Sardar Singh Kakraliya, Sachin Gupta, Sneha Choudhary, Stanzin Diskit, Shazia Paswal, Devanshi Pandit and Sheikh Saima Khushboo

Abstract:
The experiment was conducted under in vitro evaluation of different botanicals viz., Azadirachta indica (neem leaf), Azadirachta indica (neem seed cake), Allium sativum (garlic), Aloe vera (aloevera), Lanatana camara (lantana), Ocimum sanctum (tulsi) and Azadirachta indica + Lantana camara were used of ethanol extract of different concentrations 5 and 10 per cent were tested by poisoned food technique. The results reveals that Azadirachta indica was found most effective and resulted in maximum inhibition of 33.18 and 63.04 per cent followed by Azadirachta indica + Lantana camara which showed 30.71 and 55.95 per cent inhibition at 5 and 10 per cent concentrations, respectively. Plant extracts showing maximum efficacy and minimum inhibition against Veticillium fungicola were further evaluated against Veticillium fungicola infection in mushroom crop room (in-vivo test). In in-vivo study, the polybags which receive Azadirachta indica showed maximum mean disease control (55.48%) and exhibited minimum mean disease incidence (28.92%). Ecofriendly management practice, i.e., use of botanicals was studied both in vitro and in vivo which gave batter results and these practices can be economical, long lasting and free from residual side effects.