Vol. 11, Issue 1 (2022)

Author(s):
Manju Kumari Choudhary

Abstract:
Fifteen sorghum (Sorghum bicolor L.) genotypes were evaluated for genetic diversity using RAPD markers. 17 primers were screened, out of which 14 primers generated total 100 bands with average level of polymorphism 93%. Based on the RAPD markers, dendrogram was constructed using the UPGMA method. The similarity coefficient ranged from 0.31 to 0.76 with an average of 0.53. The dendrogram clearly divided the 15 genotypes into six main clusters. The highest similarity coefficient (0.76) was recorded between ES-5 (G11) and EJ-4 (G-12) while the lowest similarity coefficient (0.31) was recorded between E-2 (G1) and CSV-23 (G9) and E-5 (G3) and CSV-2 (G9). Present investigation illustrates considerable potential of RAPD markers for estimating genetic diversity.