Vol. 10, Issue 9 (2021)

Author(s):
Neha Banta, Ritika Singh and Nageswer Singh

Abstract:
The protein content and SDS-PAGE of grain storage proteins in twelve genotypes of three different cereals (wheat, barley and oats) were studied. Average grain protein (%) of wheat, barley and, oats was 10.85, 10.20 and 11.09 respectively. The twelve genotypes were clearly differentiated based on the protein banding patterns generated through SDS-PAGE. The predominant proteins of cereals resolved into three groups of different molecular weight range (high, low and mid). Interestingly, it was observed that there is good distribution of mid-range molecular weight protein (75 – 25 kDa) in cereals, whereas heavier proteins (>75 kDa) were absent in all the genotypes of wheat, barley and oats. The range of molecular weight of different proteins was observed to be 25 to 71 kDa. The dendrogram obtained using UPGMA method exhibited two major clusters. Wheat and barley were present in one cluster whereas, oat genotypes were placed in a separate cluster. The clustering pattern exhibited higher similarities among the genotypes. The similarity indexes among the different genotypes of cereals were 57 per cent, 63 per cent and 71 per cent respectively.