The present study was carried out to evaluate the in vitro
anti-inflammatory activity of hydroethanolic extract of leaves of Clerodendrum infortunatum
L. plant. The HRBC membrane stabilizing activity and inhibition of protein denaturation (anti-arthritic activity) activity was undertaken using freshly drawn human blood and bovine serum albumin, respectively.
Preliminary phytochemical analysis showed presence of Carbohydrates, Glycosides, Tannins, Phenolic compounds and Flavonoids in 70% hydroethanolic extract of leaves of C. infortunatum. The extract at 10, 20, 40, 80 and 100 μg/ml concentrations showed 46.67, 49.67, 48.33, 56.00 and 62.67 percent of membrane stabilization, respectively. At the same concentrations of extract the percent inhibition of protein denaturation was 25.71, 32.5, 46.07, 57.5 and 65.36, respectively.
Many biologically active phytoconstituents including flavonoids plays a major role in neutralizing the oxidative damage and reduces inflammatory responses. The presence of such pharmacologically active principles including flavonoids in Clerodendrum infortunatum plant may be responsible for demonstration of in vitro anti-inflammatory activity which supports the use of this plant in traditional system of medicine in inflammatory conditions.