Vol. 10, Issue 9 (2021)

Author(s):
NS Chavan, Dr. SS Kale and VS Deshmukh

Abstract:
The present experiment was laid out in completely randomized design with 10 different treatments and 3 replications. The 10 different treatments involves use of different concentrations of cytokinin 6-banzylaminopurine (BAP) in Murashinge and skoog media ranging from 0 to 9 mg/lt. The experiment conducted successfully established the in vitro culture of nodal explant of green bamboo Bambusa Balcooa. The sterilization protocol was also successful to avoid contamination. Use of bavisitin, tween 20 and ethanol is enough for disinfection of explant. It was observed that the BAP concatenationof 4mg/lt and 5 mg/lt is best for initiation of shoot bud i.e. breaking dormancy of shoot bud compared to all other treatments. Moreover it was observed that BAP concentration 4mg/lt induces most number of shoots per explant. This treatment T₅ is _also useful for increasing shoot length and number of leaves per explant after 21 days cycle.