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Vol. 10, Issue 8 (2021)

Rice hybrid purity testing at molecular level for development of F2 mapping population

Savankumar N Patel, VP Patel, KG Modha and VB Parekh
Microsatellite markers are most commonly use in fingerprinting, for assessing variation within parental lines and testing the genetic purity of hybrid seed in rice at molecular level. The present investigation was undertaken to assess the hybridity of experimental F1 plants derived from the cross between two lines naming NVSR 2179 and NVSR 2803 having contrasting characters for yield and its attributes. Both of the parents screened using five SSR (Simple Sequence Repeat) markers to identify polymorphic marker. The SSR marker naming RM225 found to be polymorphic between two parents. Total 46 F1s and parents were screened using polymorphic marker RM225 using PCR method. The results showed all the 46 plants are true hybrid at molecular level and can be used for development of mapping population. Screening of 100 individuals of F2 mapping population using same SSR markers further conformed its purity base on mendalian segregation pattern of marker alleles.
Pages: 1358-1362  |  165 Views  45 Downloads

The Pharma Innovation Journal
How to cite this article:
Savankumar N Patel, VP Patel, KG Modha, VB Parekh. Rice hybrid purity testing at molecular level for development of F2 mapping population. Pharma Innovation 2021;10(8):1358-1362.
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