CD163 expression in monocyte derived macrophages of Landrace and Ghurrah breeds confers susceptibility to Classical swine fever virus infection
Harish Kumar KP, Shikha Saxena, Richa Arora, Raj Kumar Singh and Amit Kumar
Classical swine fever (CSF) is a highly infectious and usually fatal disease of swine caused by the classical swine fever virus (CSFV), a member of the pestivirus genus and the Flaviviridae family. CSFV is a spherical viral particle with a 40-60 nm in size and its nucleocapsid having positive-strand RNA genome of 12.3 kb. In our present study we identified CD163 receptor expression in Classical swine fever virus infected porcine monocyte derived macrophages among two breeds of swine (Ghurrah and Landrace). The expression of CD163 which is the receptor of monocytes/macrophages was found to be upregulated in Landrace as compared to Ghurrah. Because, there is correlation between permissiveness of CSF virus with the CD163 receptor expression, the viral copy number was assessed by real-time PCR in these two breeds. So, we found high viral load in Landrace which signifies more virus permissiveness or increased infectiousness in mature macrophages of Landrace. This is the first study for the comparison of CD163 receptor expression with CSFV viral load in Ghurrah and Landrace.
How to cite this article:
Harish Kumar KP, Shikha Saxena, Richa Arora, Raj Kumar Singh, Amit Kumar. CD163 expression in monocyte derived macrophages of Landrace and Ghurrah breeds confers susceptibility to Classical swine fever virus infection. Pharma Innovation 2021;10(8):758-762.