Assessment of genetic diversity in an elite set of restorer lines of Helianthus annuus L using PCR-based method
RN Dhawale, MD Mahalle, KM Sharma and SD Karnewar
Genotyping of ten sunflower cultivars were done by 112 SSR primers generated differential banding patterns among different lines. All SSR primers were able to generate with an average 29.74 amplicons per primer. Out of total 3331 amplicons 21.08% were showed polymorphism among lines with 06.21 average numbers of polymorphic bands. Ha806-ar primer was the most informative primer as it generated the highest number of polymorphic amplicons while Ha357-ar stood least informative by generated less numbers of polymorphic amplicons. The lowest numbers of amplified fragments were generated by primer Ha360-ar. A perusal of dendrogram analyzed with a Euclidian distance of 0.26. The diversity in DNA level within 10 cultivars was indicating that sunflower cultivars did not show much variation at DNA level. Two groups (LSF-10, GMU-479 and LDM-72, LDM-02) were identified within which all the sunflower cultivars share 100% similarity for molecular (SSR) level, and also one group was also none of single line were identified with 100% dissimilarity.
How to cite this article:
RN Dhawale, MD Mahalle, KM Sharma, SD Karnewar. Assessment of genetic diversity in an elite set of restorer lines of Helianthus annuus L using PCR-based method . Pharma Innovation 2021;10(8):629-633.