Vol. 10, Issue 7 (2021)

Author(s):
Saurabh, Sushant Srivastava, Bhoopendra Singh, Kabir Alam, Rabindra Kumar, Rajesh Kumar and Mayank Kumar Dubey

Abstract:
The present study was carried out at Deep Frozen Semen Laboratory of C.V.Sc. & A.H., A.N.D.U.A. & T., Kumarganj, Ayodhya (U.P.) with the aim to evaluate the effect of ascorbic acid on cryopreservation of Murrah bull semen. Six ejaculate were collected from each 8 mature Murrah bull at frozen semen laboratory, were used to evaluate the effect of antioxidant at post-dilution and at post thaw stage. The semen sample was extended with Tris-Egg-Yolk-Citric-acid-Fructose-Glycerol (TEYCAFG) extender and was divided into three groups: Group 1 without any additive/ control (T₀), group second contain dilutor with ascorbic acid (.20mg/ml) treatment group (T₂) and third group’s semen contain dilutor with ascorbic acid (.50mg/ml) treatment group (T₃). Significant differences (p<0.05) was observed between the treatments group and control of post diluted and post thaw semen. Progressive motility, sperm viability, sperm abnormality, acrosomal integrity and hypo-osmotic swelling test (HOST) was evaluated at both post-dilution and post-thaw stage. Ascorbic acid fortified groups (T₁ & T₂), recorded significant (p<0.05) improvement in progressive motility, live spermatozoa, acrosomal integrity and HOST positive spermatozoa, while significant (p<0.05) decreased sperm abnormalities in post-thawed semen.