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Vol. 10, Issue 7 (2021)

Identification of canine parvovirus variants by polymerase chain reaction (PCR) based restriction fragment length polymorphism (RFLP)

Author(s):
Vivek Subramanian, G Selvaraju, S Saravanan and KM Palanivel
Abstract:
The research study was conducted to identify the canine parvovirus variants (CPV 2a, CPV 2b, & CPV 2c) by using type specific primers based polymerase chain reaction (PCR) followed by Restriction fragment length polymorphism (RLFP). Totally 180 numbers of faecal samples were collected in the form of faecal swabs from the domesticated dogs. Type specific primer pair CPV 2ab and CPV 2b were used in PCR to identify the CPV type 2a and CPV type 2b followed by RFLP using the enzyme Mbo II to differentiate the CPV type 2c from CPV type 2b after recognize selectively the sequence GAGAA at 4062 – 4064. Out of 180 samples screened, 80 samples were found to be positive to CPV-2ab primers and 14 samples were positive to CPV-2b primers and indicated that 66 samples were positive to CPV – 2a. PCR-RFLP could not detect any CPV – 2c strain from this study.
Pages: 1165-1168  |  180 Views  48 Downloads


The Pharma Innovation Journal
How to cite this article:
Vivek Subramanian, G Selvaraju, S Saravanan, KM Palanivel. Identification of canine parvovirus variants by polymerase chain reaction (PCR) based restriction fragment length polymorphism (RFLP). Pharma Innovation 2021;10(7):1165-1168.
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