Standardization of the dosage of liquid formulation of Bacillus amyloliquefaciens and Pseudomonas spp. for sesame seed treatment and assessing the spermosphere colonization
M Muthamilan and S Balakrishnan
A two days old liquid formulation of B. amyloliquefaciens containing 83.67 x 10-10 cfu / 0.1 ml was used as such in different quantities for treating one kg of sesame seed i.e. 10 ml/kg, 75 ml/kg, 100 ml/kg and 120 ml/kg of seeds and stored separately at room temperature for seventy five days. Then these treated seeds from each treatment were washed with known quantity of sterile distilled water individually and assessed the spermosphere population of B. amyloliquefaciens separately. The suspension collected from the seeds stored for seventy five days after treating with liquid formulation of B. amyloliquefaciens @ 10 ml/kg recorded 2.67 x 10-9 cfu / 0.5 ml suspension at 48 hours after plating. In another experimental study when the seeds were used ninety days after seed treatment for conducting roll towel method clearly revealed that the seed treated with liquid formulation of Pseudomonas spp. @ 20 ml/kg seed recorded highest shoot and root length of ten days old seedlings by recording 12.86 and 9.92 cm, respectively and the seeds treated with liquid formulation of B. amyloliquefaciens @ 20 ml/kg was found to record increased shoot and root length of seedlings by 12.42 cm and 9.34 cm respectively. These two treatments were statistically on par with each other
How to cite this article:
M Muthamilan, S Balakrishnan. Standardization of the dosage of liquid formulation of Bacillus amyloliquefaciens and Pseudomonas spp. for sesame seed treatment and assessing the spermosphere colonization. Pharma Innovation 2021;10(6):91-94.