Vol. 10, Issue 5 (2021)

Author(s):
Jayasri A, Narasa Reddy GV, Setty OH and Ravinder Reddy V

Abstract:
Aflatoxin and Ochratoxin A are produced from Aspergillus parasiticus and Aspergillus ochraceus respectively and quantified using thin layer chromatography. The toxins, aflatoxin at the rate of 1 ppm and ochratoxin at the rate of 2 ppm, were mixed in broiler diet. Activated charcoal (0.4%) and/or lyophilized yeast culture (0.2%) were added as adsorbents. Broiler chicks were divided into four groups with four replicates of eight birds each in a completely randomized design based on diets - Group 1- basal diet (control), Group 2- basal diet added with aflatoxin (1 ppm) and ochratoxin (2 ppm), Group 3- basal diet added with aflatoxin (1ppm), ochratoxin (2 ppm) and activated charcoal (0.4%) and Group 4- basal diet added with aflatoxin (1ppm), ochratoxin (2 ppm), activated charcoal (0.4%) and lyophilized yeast culture (0.2%). These four diets were fed to four groups of broiler chicks for 6 weeks. The body weight gains, feed consumption and feed conversion ratio were significantly (P< 0.01) lower in group 2 fed with aflatoxin and ochratoxin. These values were significantly improved in group 3 fed with aflatoxin, ochratoxin and activated charcoal. Further significant improvement was recorded in group 4 on diet containing aflatoxin, ochratoxin, activated charcoal and lyophilized yeast culture but these values were significantly lower than that of control group. The result of the study indicates that mixture of adsorbents (activated charcoal and lyophilized yeast) were more effective in reducing the effect of aflatoxin and ochratoxin compared to activated charcoal alone.