Vol. 10, Issue 5 (2021)

Author(s):
Chungada AS, Gahukar SJ, Akhare AA, Behere GT, Solanke AU and Patil SR

Abstract:
In the present investigation, A total of twenty-one SSR primers were used to evaluate the genetic diversity of six citrus species. Seven primer pairs failed to show any amplification thus revealing no bands (null allele) in all the genotypes. Fourteen SSR markers exhibited polymorphism and showed high levels of allelic diversity. A total of 139 amplicons were amplified by 14 polymorphic SSR loci and the number of amplicons ranged from 5 to 18 with an average of 9.92 amplicons per locus. Only one primer i.e. SSR-5 shown 100 % polymorphism, whereas, 87 % polymorphism was observed in SSR-2 and 67 % polymorphism was detected in SSR-3, SSR-9, SSR-12 and SSR-13; showed an average 66.14 % polymorphism percent. The PIC value of 14 microsatellite loci ranged from 0.41 to 0.86 with an average value of 0.59. For each marker, the maximum PIC value was observed in marker SSR-5 i.e. 0.86 and the minimum was in SSR-11 i.e. 0.41. The highest similarity was found between Alemow and Galgal with a correlation coefficient value of 0.64. The lowest similarity coefficient was observed between Jambhiri and Galgal with a similarity coefficient value of 0.44.