Vol. 10, Issue 5 (2021)

Author(s):
Shruti, AH Ahmad, Sanjeev Agarwal, Ashutosh Dubey and AK Verma

Abstract:
Berberis lyceum Royle is a traditional medicinal plant of the Shivalik range of Himalaya, India, and reported first time in vitro anti-inflammatory potentials in the root, stem, and leaves against denaturation of protein. The different concentration of B. lyceum Royle extract, pure berberine, and diclofenac as standard was incubated with albumin in controlled experimental conditions and subjected to determine the absorbance and to measure their viscosity. The assay is based on the denaturation of protein upon the heat treatment as the anti-inflammatory agent will stabilize or prevent the protein from denaturation. A concentration-dependent inhibition of protein (albumin) denaturation by the B. lyceum Royle and berberine was found in both. However, hydroethanolic extract B. lyceum Royle was found to more effective when compared to pure berberine and diclofenac. High anti-inflammatory effect of hydroethanolic extract due to the synergistic effect of other phytoconstituents present in B. lycium extract along with berberine. Therefore from the present study, we can conclude that both berberine and B. lyceum Royle possess anti-inflammatory activity.