Effect of different Anti-oxidants on the Acrosome integrity and Sperm membrane Integrity (HOST) of Deccani ram sperm preserved at 5 oc
Anil Manga, K Venkataramana and K Muralimohan
A study was conducted to assess the influence of Ascorbic acid, Butylated hydroxy toluene (BHT) and Vit E on semen quality parameters of Deccani rams. Semen was collected from eight Deccani rams twice a week and one twenty eight collections (sixteen collections per ram) were utilised in this study. After collection of semen sample, each semen sample diluted 1:3 with Tris citrate fructose egg yolk (TCFEY) extender then split into four equal parts first one added with Ascorbic acid (2mg/ml), second part added with Butylated hydroxy toluene (BHT) at the rate of 2mM/ml, third part added with Vitamin E at the rate of 2mg/ml and the fourth part was considered as control without any addition. And acrosome integrity and HOST (Hypo osmotic swelling test) positive sperms were evaluated at an interval of 24, 48 and 72hrs at refrigerated temperature (5 °c). The results were significantly (P< 0.05) better in extender supplemented with BHT, Vit E group than the Ascorbic acid and control group. But Ascorbic acid supplemented group better when compared with those of control diluent at different hours of incubation. The results revealed that supplementation of Ascorbic acid, BHT and Vit E to Tris citrate fructose egg yolk (TCFEY) extender improve semen quality in Deccani rams.
How to cite this article:
Anil Manga, K Venkataramana, K Muralimohan. Effect of different Anti-oxidants on the Acrosome integrity and Sperm membrane Integrity (HOST) of Deccani ram sperm preserved at 5 oc. Pharma Innovation 2021;10(4):26-29.