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Vol. 10, Issue 4 (2021)

Assessment of Hybrid Purity in Maize (Zea mays L.) Using RAPD and SSR Markers

Author(s):
NA Shinde, AA Bharose, DK Sarode, RS Swathi, PA Pimpale and SS Shinde
Abstract:
Maize (Zea mays L.; 2n=20) is third most important cereal crop and referred as the “Queen of Cereals” and “Miracle Crop” due to its high productivity potential as compared to the other cereals. Maize is native to Mexico and Central America. Maize is the most important crops in the world that can be grown in diverse seasons, ecologies it is used mainly for human food, animal feed and industry. This work was carried out to assess the hybrid purity of maize hybrid Hyd.18227 × Hyd.10306 (Tall) and their parental line Hyd.18227 (Dwarf) and Hyd.10306 (Tall) using RAPD and SSR markers. DNA extracted from young leaves and PCR were conducted using 24 RAPD and 18 SSR markers. Out of fifteen RAPD markers and five SSR makers showed the polymorphism between the maize parental lines and remaining primer produced monomorphic banding pattern. The SSR primer Umc-1858 identified 64 % hybrid seeds of total cross.
Pages: 870-874  |  330 Views  144 Downloads


The Pharma Innovation Journal
How to cite this article:
NA Shinde, AA Bharose, DK Sarode, RS Swathi, PA Pimpale, SS Shinde. Assessment of Hybrid Purity in Maize (Zea mays L.) Using RAPD and SSR Markers. Pharma Innovation 2021;10(4):870-874.
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