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Vol. 10, Issue 4 (2021)

Isolation, Enrichment and characterisation of Caprine bone marrow derived mesenchymal stem cells

Author(s):
Sonam Rani, SD Kharche, SP Singh, Chetna Gangwar, D Jena and Ashok Kumar
Abstract:
Mesenchymal stem cells are now- a-days treated as the cornerstone of the cellular therapy and tissue engineering arena. These omnipresent cells can be isolated from a wide range of sources and can be differentiated and trans-differentiated into many cell types based on specific cellular command. In this study, we had isolated bone marrow derived mesenchymal stem cells from 1-2 years old apparently healthy goats. The heterologous cell population then subjected to magnetic activated cell sorting against CD73+, CD90+, CD105+, CD271+ and CD34- to exclude mesenchymal stem cells from haematopoietic stem cells. The cells were then cultured and incubated at 37 °C in humidified atmosphere with 5% CO2. After 3 passages the cells were tested for alkaline phosphatase activity. Furthermore, the cells were also evaluated based on their morphometric analysis.
Pages: 166-169  |  482 Views  94 Downloads


The Pharma Innovation Journal
How to cite this article:
Sonam Rani, SD Kharche, SP Singh, Chetna Gangwar, D Jena, Ashok Kumar. Isolation, Enrichment and characterisation of Caprine bone marrow derived mesenchymal stem cells. Pharma Innovation 2021;10(4):166-169. DOI: 10.22271/tpi.2021.v10.i4c.5912
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