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Vol. 10, Issue 2 (2021)

Cytotoxic and antiproliferative potential of methanolic extracts of Asparagus racemosus in MDAMB231 cells

Author(s):
G Dhanusha, S Sujith, AR Nisha, KK Aathira and JS Haima
Abstract:
The present study was carried out to evaluate the cytotoxic and antiproliferative activity of methanolic extract of Asparagus racemosus in MDAB-231 cells. The qualitative phytochemical analysis of the extract revealed the presence of steroids, alkaloids, flavonoids, glycosides, saponins and diterpenes. MDAMB-231 cells were maintained in RPMI media containing 10 per cent serum and 1 per cent antibiotic and antimycotic solution. The cells were harvested and seeded to 96 well plate at 5×103 cells/mL, incubated for 24 hours at 37˚C with 5 per cent CO2. The cells were treated with 160, 80, 40, 20 and 10µg/mL of the extract for 24 hours and viability was accessed using MTT Assay. Also cells were plated in 6 well plates at concentrations 3×105 cells/mL and exposed to 80, 40, 20 and 10µg/mL of the extract of Asparagus racemosus for acridine orange ethidium bromide (AO/EB) staining. There was a dose dependent decrease in viability of cells exposed to methanolic extracts of Asparagus racemosus with a viability of 15.55+0.193 per cent for cells treated with 160µg/mL. The IC50 was found to be 91.36+ 0.87 µg/mL. The AO/EB staining revealed that most of the cells were dead in extract treated with 80µg/mL where more than 90 per cent of cells were live when treated with 10µg/mL of the extract.
Pages: 355-358  |  901 Views  60 Downloads


The Pharma Innovation Journal
How to cite this article:
G Dhanusha, S Sujith, AR Nisha, KK Aathira, JS Haima. Cytotoxic and antiproliferative potential of methanolic extracts of Asparagus racemosus in MDAMB231 cells. Pharma Innovation 2021;10(2):355-358. DOI: 10.22271/tpi.2021.v10.i2e.5693
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