Vol. 10, Issue 12 (2021)

Author(s):
Joshi AJ, Parmar VL, Zeal R Acharya, Udutha JV and Madhuri M

Abstract:
The present investigation on was carried out in south Gujarat heavy rainfall region of India. Callus derived from sugarcane variety CoN – 13073 subjected for mutagenic treatment varying in different concentration as well as time period. Callus culture of 25 - 30 days old white globular form is considered a suitable material to undergo mutagenic treatments. Among the mutagenic treated calli, maximum survival per cent was observed in EMS 0.2% + 60 min treatment. Maximum regeneration per cent (78.2%) was found in explants treated with treatment MMS 0.3% + 30 min. In case of the number of days for shoot formation, callus treated with EMS 0.2% + 60 min registered a maximum of 15.20 days. The maximum numbers of multiple shoots were observed in MMS 0.2% + 60 min (30.4). Time factor found to be highly significant with a wide range of mean performance. The effect of the mutagenic agent was found to be succeeding to create variability at a lower concentration for lower time intervals for most of the characters under study. In the present study EMS, 0.2% in addition to 0.3%, treatments were most effective. And the effectiveness decrease with the increase in the time interval.