Vol. 10, Issue 12 (2021)

Author(s):
Supriya Ambawat, C Tara Satyavathi, RC Meena, Rajbala Meena, Vikas Khandelwal, Subaran Singh and Rajesh Geela

Abstract:
Pearl millet [Pennisetum glaucum (L.) R. Br.] is a major cereal crop grown mainly in the arid and semi-arid regions of Asia and Africa. DNA fingerprinting is mandatory for registration of newly developed hybrids and varieties with National Bureau of Plant Genetic Resources (NBPGR), New Delhi and Protection of Plant Varieties and Farmers' Rights Authority (PPV&FRA). In the present study, two advanced hybrid entries of pearl millet of third year testing viz. MH 2423 (NBH5929) and MH 2439 (86M80) were analyzed for DNA fingerprinting along with two checks (KBH108 and 86M86) using 36 SSR markers. Out of the 36 primers used, 26 SSR primers (72.2%) were found polymorphic with MH 2423 (NBH5929) and 28 SSR primers (77.8%) were found polymorphic with MH 2439 (86M80). The SSR profiles were analyzed based on pattern of bands and a unique profile was obtained for both the hybrids with amplicon size ranging between 100 to 500 bp. Thus, the SSRs very well differentiated the two hybrids generating a unique DNA fingerprinting profile for both the hybrids. The identified makers can prove useful further for genetic purity testing, utilization for identification of the diverse germplasm and future DNA fingerprinting studies.