Vol. 10, Issue 12 (2021)

Author(s):
Rajeshwari S Durgad, Raviraja Shetty G, Rajasekharan PE, Harsha R, Ganapathi M and Sadashiv Nadukeri

Abstract:
An investigation was undertaken on tissue culture and In-vitro conservation studies in Operculina turpethum (L.) Silva Manso. at the Division of Floriculture and Medicinal Crops, ICAR- Indian Institute of Horticultural Research, Bengaluru during 2020-2021. The experiment was laid out in Completely Randomized Design (CRD) and replicated thrice. Shoot was multiplied using nodal explants cultured on MS medium supplemented with various combinations of growth regulators like BAP, Kinetin and NAA. Among the different combinations, BAP (1 mg/l) + NAA (0.5 mg/l) recorded maximum number of shoots (3.25±0.05) and leaves per explant (4.95±0.04) with minimum days for shoot initiation (9.15±0.06). Whereas, the highest shoot length (4.65±0.05 cm) was noticed on BAP (1 mg/l). The satisfactory results after three months of In-vitro conservation were obtained using BAP (1 mg/l) + NAA (0.5 mg/l) in terms of high survival per cent (100), maximum number of shoots (3.30±0.02) and leaves per explant (4.68±0.04). Hence, BAP (1.0 mg/l) + NAA (0.5 mg/l) and BAP (1.0 mg/l) can be considered as the best treatment combination for In-vitro shoot multiplication of O. turpethum. Further, the protocol can be utilized for effective conservation, utilization and multiplication of this valuable threatened medicinal plant.