Vol. 10, Issue 10 (2021)

Author(s):
K Jeeva, N Chatterjee, AK Bera, S Maiti, Hira Ram, PS Banerjee, TK Goswami, PJ Das, JK Chamua, SM Deb and D Bhattacharya

Abstract:
T. canis was collected from dogs in yak tracts of Arunachal Pradesh and Tamil Nadu. T. cati was isolated from cats of Arunachal Pradesh; T. vitulorum was collected from yaks of Arunachal Pradesh, mithun of Nagaland and cattle of West Bengal. DNA extraction was done by following conventional liquid-liquid extraction method, purity of genomic DNA and quantification of DNA was done by spectrophotometric analysis and for conducting RAPD-polymerase chain reaction (RAPD-PCR), total of 25 arbitrary primers were used in appropriate cycling condition. RAPD fingerprinting was conducted using 25 arbitrary primers and only 9 primers could yield scorable bands which differentiate three species of Toxocara which could be appreciated on the basis of simple matching co-efficient (SMC) and distance matrix after third clustering step based on single lineage. T. canis collected from two different geographical locations exhibited indistinguishable RAPD profile. Further, T. vitulorum collected from yak, mithun and cattle of three different geographical locations had similar RAPD profile which indicated genetic similarity of parasites irrespective of host assemblages and geographical locations. This study seems to be the seminal Indian effort on comparative genetic profile of three Toxocara Spp., of different host species and varied geographical locations.